Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals
نویسندگان
چکیده
Controlling crystal nucleation is a crucial step in obtaining high quality protein crystals for structure determination by X-ray crystallography. Carbon nanomaterials (CNMs) including carbon nanotubes, graphene oxide, and carbon black provide a range of surface topographies, porosities and length scales; functionalisation with two different approaches, gas phase radical grafting and liquid phase reductive grafting, provide routes to a range of oligomer functionalised products. These grafted materials, combined with a range of controls, were used in a large-scale assessment of the effectiveness for protein crystal nucleation of 20 different carbon nanomaterials on five proteins. This study has allowed a direct comparison of the key characteristics of carbon-based nucleants: appropriate surface chemistry, porosity and/or roughness are required. The most effective solid system tested in this study, carbon black nanoparticles functionalised with poly(ethylene glycol) methyl ether of mean molecular weight 5000, provides a novel highly effective nucleant, that was able to induce crystal nucleation of four out of the five proteins tested at metastable conditions.
منابع مشابه
Investigation of Different Stages of Aluminum Fluoride Crystal Growth
Crystallization of Aluminum fluoride at atmospheric pressure has been considered. Structure, size and shape of crystals formed during the crystallization process have been investigated. By applying the direct analysis method for the existed aluminum in solution, the aluminum fluoride nucleation process has been detected as a concentration valley at the outset of crystallization process. The...
متن کاملNew hybrid nanomaterial derived from immobilization of a molybdenum complex on the surface of multi-walled carbon nanotubes
In this work, we report a new well dispersed molybdenum complex attached through the mediation of aminopropylsilyl groups on the surface of multi-walled carbon nanotubes (MWCNTs). The prepared hybrid nanomaterial was characterized with different physicochemical methods such as Fourier transform infrared and atomic absorption spectroscopies, transmission electron microscopy, energy-dispersive X-...
متن کاملEFFECT OFMASS TRANSPORT STABILITYAND UNDERCOOLING ON NUCLEATION OF ZNS CRYSTALS IN CLOSED AMPOULE CVT
متن کامل
Effect of impurities on the optical properties of KTP single crystals grown from flux
In the present work, KTP crystals have been grown by spontaneous nucleation technique in flux medium using K6P4O13 flux. 0.4-1 °C/h cooling rates were applied in the spontaneous nucleation process. The presence and amount of impurities has been determined by using XRF. The optical transmission spectra of impure KTP crystals in the UV–visible region are discussed. The transmission cut-off is cle...
متن کاملElectrochemical Determination of Neonicotinoid Insecticide Clothianidin by Nanomaterial based Disposable Electrode
Disposable, selective and low-cost carbon nanotube modified electrodes were fabricated for the electrochemical detection of neonicotinoid insecticide, Clothianidin (CLT). Almost 2 folds’ increase at Clothianidin reduction signal was obtained by using single-walled carbon nanotube modified electrodes (SWCNT-PGE) compared to unmodified electrodes. These nanomaterials modified electrodes developed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2016